[3view] 3view Digest, Vol 29, Issue 3

Beckett, Alison A.J.Beckett at liverpool.ac.uk
Thu Aug 23 12:27:40 CEST 2018


Morning,

I have the same issue with my 3view/FEI system.

It happened with the previous software but is more frequent since changing to GMS3. As Louise said, re starting the system is the only way to correct the fault for me.

It manifests in two ways I have seen. The knife moves normally but the stage no longer moves the sample up between cuts. Also but less often, the knife seems to reset itself to a position where it no longer reaches the sample during cut phase.

Best,

Alison

*********************
Alison Beckett
EM Unit
Dept of Cellular & Molecular Physiology
Institute of Translational Medicine
University of Liverpool
Crown Street
Liverpool
L69 3BX

Tel: +44 151 794 5461
http://www.liverpool.ac.uk/emunit
http://www.threshersharkproject.org
https://uk.virginmoneygiving.com/AlisonBeckett

On 23 Aug 2018, at 11:00, <3view-request at open-em.org<mailto:3view-request at open-em.org>>
 wrote:

Send 3view mailing list submissions to
3view at open-em.org<mailto:3view at open-em.org>

To subscribe or unsubscribe via the World Wide Web, visit
http://open-em.org/mailman/listinfo/3view
or, via email, send a message with subject or body 'help' to
3view-request at open-em.org

You can reach the person managing the list at
3view-owner at open-em.org

When replying, please edit your Subject line so it is more specific
than "Re: Contents of 3view digest..."


Today's Topics:

  1. Re: knife moving problem (Louise Hughes)


----------------------------------------------------------------------

Message: 1
Date: Thu, 23 Aug 2018 08:08:07 +0100
From: Louise Hughes <lhughes at brookes.ac.uk>
To: Mailing list for Gatan 3View Serial Block-Facing
<3view at open-em.org>
Subject: Re: [3view] knife moving problem
Message-ID:
<CAP_aGBSmbGxeeb2_LYAWtsCBV6Yqvh=PW1NNvC9gcCvyoR8cyQ at mail.gmail.com>
Content-Type: text/plain; charset="utf-8"

Dear All,

I am on GMS3 and have had the same issues with the timeout message several
times and also no slicing upon approach for several microns. It is a
periodic error and was usually corrected by restarting the system. I think
it is a communication issue. It is worth talking to Gatan about this. After
we had several incidents they sent an engineer out and they replaced one of
the USB ports or connectors (if I recall correctly). It doesn't seem to
have happened since, although some of the other communication issues with
the Zeiss computer still seem to occur (this may not be a Gatan issue,
however).

This seems to be a separate issue than the noises you are hearing and the
seizure of the system. Have you contacted Gatan?

I hope this is helpful.

Regards,
Louise

On 23 August 2018 at 06:29, Belevich, Ilya <ilya.belevich at helsinki.fi>
wrote:

Hi Gosia,

I agree with Joergen, it is difficult to target the problem. Especially,
since it happened only few times during the year. Your events also may be
due to certain accidents, such as miscommunication between DM and the knife
controller (perhaps, in the second case with the error message: 3View
record aborted:Timeout moving knife position to NEAR).

With the other symptoms, I also had a weird case when I suppose to cut but
nothing was going on for several microns. Basically, I just opened the
chamber, lowered the stage and re-approached. This accident never happened
again.

You may try to check the contact that Joergen suggested (I am don?t know
where it is myself). I would just keep monitoring the system and if the
same accidents occur 3 times or more start to worry. If the event is not
reproducible it will be very difficult to tackle the problem and you might
ending up in replacing part after part.



Are you on DM2 or GMS3?



Best regards,

Ilya



--------------------------------------------------------------

Ilya Belevich, PhD

Senior researcher, Electron Microscopy Unit

Institute of Biotechnology, PL56 (Viikinkaari 9)

University of Helsinki, 00014 FINLAND

phone: +358-2-941-59497   email: ilya.belevich(AT)helsinki.fi

web:   http://www.biocenter.helsinki.fi/~ibelev



*From:* 3view-bounces at open-em.org [mailto:3view-bounces at open-em.org] *On
Behalf Of *Malgorzata Sliwinska
*Sent:* Wednesday, August 22, 2018 6:20 PM
*To:* Mailing list for Gatan 3View Serial Block-Facing <3view at open-em.org>
*Subject:* Re: [3view] knife moving problem



Hi Joergen,

Thank you for your suggestions. Would it be possible for you to send me a
picture showing what you mean by electric wire which bend over time? I
would like to try to bend it back. I hope it is not a motor as I am able to
cut, after error I start new scan and it is working fine for two hours
now... How to check if it is because of cutter piezo?

Generally can I do everything myself  or I need to call Gatan?

Thanks a lot

Gosia















Ma?gorzata ?liwi?ska, PhD

Laboratory of Imaging Tissue Structure and Function

Nencki Institute of Experimental Biology Polish Academy of Sciences

3 Pasteur Street, 02-093 Warsaw, Poland

Phone: +48 22 589 2502

www.nencki.gov.pl

W dniu 2018-08-22 16:34, Joergen Kornfeld napisa?(a):

Hi Gosia,

it is a bit hard to diagnose for me from the description, but it might be
the "cutter motor" or the detection mechanism that tests in which position
the cutter arm is. For imaging, the whole knife holding arm is moved away
from the sample (this may be what you mean by CLEAR position), but then
returned over the sample for cutting. This position is detected by an
electric wire contact, which might bend over time so that it is not
functional anymore and the cutter arm can get stuck (just bending it back
would fix it then). Another possibility is that the cutter motor itself is
broken (the entire arm with the knife does not move anymore, needs
replacement of the motor) or that the knife piezo does not work anymore
(the diamond cannot slide over the sample anymore during approach, needs
replacement of the cutter piezo).

Maybe this helps,

Joergen



Am 8/22/2018 um 4:14 PM schrieb Ma?gorzata ?liwi?ska:

Dear All,

Recently I have experienced three times a problem with knife moving in
3View.

I the middle of the scan the knife stopped cutting. The pictures were
collected, I hear clicking from the knife driver, so it seems that
everything is working but 20 consecutive pictures were the same, no
cutting. My impression is that the table with the sample was moving up but
the knife was blocked in CLEAR position. When it happened I opened the
chamber, lower the stage and approached the sample again (because I was
afraid that if the knife unblock finally, it will hit the sample and be
damaged). After restart the scan continued properly.

Today it happen again, but this time the scan was stopped automatically
and I received the message: "3View record aborted:Timeout moving knife
position to NEAR"

Also from one year on I have been observing, that while cutting with open
chamber, around the 12-17th slice I hear the sound like  "boing" of the
metal spring (I did not observed any blockade with the open chamber but I
suspect that it can be the problem of the spring responsible for moving the
knife arm...)

Does anybody experience similar problem? Does it concern a part easily
exchangeable or is it a bigger problem? I am afraid to leave 3View working
unattended till I fix this problem ;-(

I would be grateful for any advice

Best regards

Gosia









Ma?gorzata ?liwi?ska, PhD

Laboratory of Imaging Tissue Structure and Function

Nencki Institute of Experimental Biology Polish Academy of Sciences

3 Pasteur Street, 02-093 Warsaw, Poland

Phone: +48 22 589 2502

www.nencki.gov.pl



_______________________________________________

3view mailing list

3view at open-em.org

http://open-em.org/mailman/listinfo/3view



_______________________________________________

3view mailing list

3view at open-em.org

http://open-em.org/mailman/listinfo/3view


_______________________________________________
3view mailing list
3view at open-em.org
http://open-em.org/mailman/listinfo/3view




--
Dr. Louise Hughes
Bio-Imaging Unit Researcher and  Microscopist
Department of Biological and Medical Sciences,
Oxford Brookes University,
Oxford, OX3 0BP, UK
01865 484351

Brookes e-mail: lhughes at brookes.ac.uk
Personal website: http://www.miniaturehorizons.com
Twitter: https://twitter.com/DrLouiseCHughes
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://open-em.org/pipermail/3view/attachments/20180823/01ae2042/attachment-0001.html>

------------------------------

_______________________________________________
3view mailing list
3view at open-em.org
http://open-em.org/mailman/listinfo/3view


End of 3view Digest, Vol 29, Issue 3
************************************

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://open-em.org/pipermail/3view/attachments/20180823/94c8c51e/attachment-0001.html>


More information about the 3view mailing list