[3view] movement of the 3View knife

Tobias Starborg tobias.starborg at manchester.ac.uk
Tue Oct 7 15:32:07 CEST 2014

Hi Saskia,  I have seen this at least once, though I was showing the people the system, so didn't have a chance to look into it.  I get a similar effect if I move the knife to 600µm (for example), then change my mind and try to move to 500µm without going back to the retract position.

Mine did clear initially, but restarted during approach.  I ended up restarting the 3 boxes and it was fine after that.  I've not seen it since.

No errors that I can remember, though I was a bit flustered as I had lots of people keen to see their data in the room, so my main concern was to get it working quickly rather than trouble shoot.

Not sure that helps, but at least we have more than one occurrence.

Dr Tobias Starborg
Senior Experimental Officer: 3DEM
Wellcome Centre for Cell Matrix Research
Michael Smith Building
M13 9PT
Tel: +44(0)1612755170

From: Saskia Lippens <Saskia.Lippens at irc.vib-ugent.be<mailto:Saskia.Lippens at irc.vib-ugent.be>>
Reply-To: Mailing list for Gatan 3View Serial Block-Facing <3view at open-em.org<mailto:3view at open-em.org>>
Date: Tuesday, 7 October 2014 12:27
To: "3view at open-em.org<mailto:3view at open-em.org>" <3view at open-em.org<mailto:3view at open-em.org>>
Subject: [3view] movement of the 3View knife

Hi everyone,

in Ghent we noticed that the horizontal movement of the 3View knife (from 0-1200 um) is not always occurring in 1 smooth stroke, but the knife stops for a brief moment in 2 positions. We noticed this during the approach. When we stop the cutting and restart the 'phenomenon' does not take place anymore. Of course, we want to be sure that this does not happen during the actual acquisition.
Has anyone noticed this before?

Thank you for your response,


Saskia Lippens, Ph.D.
Outreach Coordinator
FVMS Building - VIB
Technologiepark 927
9052 Zwijnaarde
Saskia.Lippens at irc.vib-UGent.be<mailto:Saskia.Lippens at irc.vib-UGent.be>

[cid:285C07E9-B1A3-459F-95D6-D3A199704CB5 at irc.ugent.be][cid:3063916E-D9FE-4A8B-A003-F05DD36EE6A1 at irc.ugent.be]

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